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Journal: Scientific Reports
Article Title: C57BL/6J mice best recapitulate fibrosis and inflammatory pathophysiology in syngeneic mouse model of endometriosis
doi: 10.1038/s41598-025-13900-9
Figure Lengend Snippet: Assessment of fibrosis and iron accumulation in ectopic lesions across strains. ( A ) Masson’s trichrome staining (MTS) revealed varying degrees of collagen deposition (blue) in ectopic lesions, with C57BL/6J exhibiting the most pronounced fibrosis, followed by substantial fibrosis in BALB/c (quantified at 69.95%), and the least in Swiss albino (57.47%). ( B , C ) Prussian blue staining showed strain-dependent iron deposition (blue precipitates, arrowheads) in the lesion stroma, with significant accumulation in C57BL/6J (score: 2.348), moderate in BALB/c (score: 1.208), and minimal in Swiss albino (score: 0.356, OD: 0.084). ( D ) Collagen Type I alpha 1 (Col1A1) ELISA indicated significant stromal fibrosis in C57BL/6J (* p < 0.05), moderate fibrosis in BALB/c (lower than C57BL/6J but higher than Swiss albino), and mild fibrosis in Swiss albino. Data are mean ± SEM, * p < 0.05 (unpaired t-test).
Article Snippet: The levels of
Techniques: Staining, Enzyme-linked Immunosorbent Assay